Lefapheng la saense ea sejoale-joale, electrophoresis ea protheine e eme e le mokhoa oa bohlokoa oa ho sekaseka le ho hlalosa liprotheine. Motheong oa mokhoa ona ho na lePolyacrylamide, motsoako o feto-fetohang o sebetsang e le mokokotlo oa li-matrices tsa gel tse sebelisoang lits'ebetsong tsa electrophoresis ea gel. Thepa e ikhethang ea Polyacrylamide e etsa hore e be sesebelisoa sa bohlokoa bakeng sa bafuputsi le bo-rasaense ba batlang ho manolla mathata a liprotheine le likamano tsa tsona.
Polyacrylamide, eo hangata e bitsoang PAM, ke polymer ea maiketsetso e entsoeng ka li-monomers tsa acrylamide. Ho tenyetseha ha eona ho ikhethang ho bakoa ke bokhoni ba eona ba ho theha liketane tse telele, e leng se fellang ka ntho e kang gel e ka amohelang boholo bo fapaneng ba limolek'hule. Thepa ena e etsa hore polyacrylamide e be mokhethoa ea loketseng bakeng sa ho theha matrices e nang le masoba e sebelisoang ho electrophoresis ea protheine.
Protheine electrophoresis ke mokhoa o arolang liprotheine ho latela tefiso le boholo ba tsona. Ka ho beha sampole ea protheine tšimong ea motlakase ka har'a matrix ea gel ea polyacrylamide, liprotheine li falla ka gel ka lebelo le fapaneng, e leng se fellang ka lihlopha tse fapaneng tse ka hlahlojoang le ho lekanngoa. Karohano ena e fana ka temohisiso ea bohlokoa mabapi le bohloeki ba protheine, ho fumana boima ba limolek'hule le boteng ba li-isoform.
Karolo ea Polyacrylamide ho Protheine Electrophoresis
Khetho ea polyacrylamide bakeng sa protheine electrophoresis e thehiloe tlhahong ea eona e ka fetoloang. Bo-rasaense ba ka fetola mahloriso a matrix ea gel ho amohela liprotheine tsa boholo bo fapaneng. Mahlakore a phahameng a etsa matrices e thata e loketseng ho rarolla liprotheine tse nyane, ha mahloriso a tlase a sebelisoa bakeng sa liprotheine tse kholo. Ho ikamahanya le maemo hona ho netefatsa hore bafuputsi ba ka fetola liteko tsa bona ho fihlela karohano le tlhahlobo e ntle ka ho fetisisa.
Polyacrylamide e le sesebelisoa sa hoFlocculant
Tšebeliso ea Polyacrylamide e feta karolo ea eona ho electrophoresis ea gel. E boetse e fumana lits'ebetso e le flocculant liindastering tse fapaneng, joalo ka kalafo ea metsi le taolo ea metsi a litšila. Joaloka flocculant, polyacrylamide e thusa ho bokella likaroloana tse emisitsoeng ka har'a metsi, e leng se nolofalletsang ho tlosoa ha tsona. Tšobotsi ena e totobatsa bokhoni bo fapaneng ba motsoako le tšusumetso e pharaletseng saenseng le indastering.
Tsoelo-pele ho Electrophoresis e Thehiloeng ho Polyacrylamide
Lilemo tsa morao tjena li bone tsoelo-pele e tsoelang pele mekhoeng ea electrophoresis e thehiloeng ho polyacrylamide. Native PAGE, SDS-PAGE, le gel electrophoresis ea mahlakore a mabeli ke mehlala e 'maloa feela ea kamoo ho ikamahanya ha polyacrylamide ho nolofalitseng nts'etsopele ea mekhoa e ikhethileng ea ho sekaseka meaho ea protheine, liphetoho tsa kamora phetolelo, le likamano. Mekhoa ena e bohlokoa haholo lipatlisisong tsa proteomics le boitekong ba ho sibolla lithethefatsi.
Lefapheng la tlhahlobo ea protheine, polyacrylamide e hlaha e le motsoalle ea tiileng, e nolofalletsang bafuputsi ho hlahloba lefats'e le rarahaneng la liprotheine. Karolo ea eona e le motheo oa li-matrices tsa gel lits'ebetsong tsa electrophoresis e ke ke ea feteletsoa. Ho tloha ho qaqiseng mekhoa ea mafu ho isa ho nts'etsopele ea mekhoa e mecha ea phekolo, electrophoresis e thehiloeng ho polyacrylamide e ntse e tsoela pele ho bopa tsoelo-pele ea saense. Ha theknoloji e ntse e tsoela pele, mohlolo ona oa maiketsetso o ka 'na oa fetoha, oa ntlafatsa kutloisiso ea rona ea liprotheine le mesebetsi ea tsona e mengata.
Nako ea poso: Phato-21-2023