Hobaneng Polyacrylamide e sebelisoa bakeng sa protheine electrophoresis

Sebakeng sa mahlale a morao-rao, protheine electrophoresis e eme e le mokhoa oa motheo oa ho hlahloba le ho khetholla liprotheine.Bohareng ba mokhoa ona ke leshanoPolyacrylamide, motsoako o nang le mekhoa e mengata e sebetsang e le mokokotlo oa matrices a gel a sebelisoang mekhoeng ea gel electrophoresis.Thepa e ikhethang ea Polyacrylamide e e etsa sesebelisoa sa bohlokoa ho bafuputsi le bo-ramahlale ba batlang ho senola ho rarahana ha liprotheine le tšebelisano ea tsona.

Polyacrylamide, eo hangata e bitsoang PAM, ke polymer ea maiketsetso e entsoeng ka li-acrylamide monomers.Ho feto-fetoha ha eona ho tsotehang ho bakoa ke bokhoni ba eona ba ho etsa liketane tse telele, e leng se hlahisang ntho e kang gel e khonang ho amohela boholo bo fapaneng ba limolek'hule.Thepa ena e etsa hore Polyacrylamide e be mokhethoa ea loketseng ho theha matrices a porous a sebelisoang ho protheine electrophoresis.

Protein electrophoresis ke mokhoa o arolang liprotheine ho latela tefiso le boholo ba tsona.Ka ho beha sampole ea protheine sebakeng sa motlakase ka har'a polyacrylamide gel matrix, liprotheine li falla ka gel ka litekanyo tse fapaneng, e leng se etsang hore ho be le lihlopha tse ikhethileng tse ka hlahlojoang le ho lekanyetsoa.Karohano ena e fana ka leseli la bohlokoa mabapi le bohloeki ba protheine, boikemisetso ba boima ba limolek'hule, le boteng ba li-isoform.

Karolo ea Polyacrylamide ho Protein Electrophoresis

Khetho ea polyacrylamide bakeng sa protheine electrophoresis e thehiloe tlhahong ea eona e ka khonehang.Bo-rasaense ba khona ho fetola bongata ba gel matrix ho amohela liprotheine tsa boholo bo fapaneng.Likhakanyo tse phahameng li theha matrices a tiileng a loketseng ho rarolla liprotheine tse nyane, athe likhakanyo tse tlase li sebelisetsoa liprotheine tse kholo.Ho ikamahanya le maemo ho etsa bonnete ba hore bafuputsi ba ka hlophisa liteko tsa bona ho fihlela karohano le tlhahlobo e nepahetseng.

Polyacrylamide joalo kaFlocculant

Tšebeliso ea Polyacrylamide e fetela ka nqane ho karolo ea eona ho gel electrophoresis.E boetse e fumana lits'ebetso e le sebaka sa marang-rang liindastering tse fapaneng, joalo ka tlhoekiso ea metsi le taolo ea metsi a litšila.Joalo ka flocculant, polyacrylamide e thusa ho kopanya likaroloana tse emisitsoeng ka har'a maro, ho thusa ho tlosoa ha tsona.Tšobotsi ena e totobatsa bokhoni bo fapaneng ba motsoako le phello e pharaletseng ho saense le indasteri.

Tsoelo-pele ho Polyacrylamide-Based Electrophoresis

Lilemong tsa morao tjena ho bone tsoelo-pele e tsoelang pele ho mekhoa ea electrophoresis e thehiloeng ho polyacrylamide.Native PAGE, SDS-PAGE, le two-dimensional gel electrophoresis ke mehlala e seng mekae feela ea kamoo ho feto-fetoha ha maemo ha polyacrylamide ho nolofalitseng nts'etsopele ea mekhoa e khethehileng ea ho hlahloba libopeho tsa protheine, liphetoho tsa morao-rao tsa phetolelo le likamano.Mekhoa ena e bohlokoa haholo lipatlisisong tsa proteomics le boitekong ba ho sibolla lithethefatsi.

Sebakeng sa tlhahlobo ea liprotheine, polyacrylamide e hlaha e le molekane ea matla, e nolofalletsang bafuputsi ho phenya lefatše le rarahaneng la liprotheine.Karolo ea eona e le motheo oa matrices a gel mekhoeng ea electrophoresis e ke ke ea fetisoa.Ho tloha ho ho manolla mekhoa ea mafu ho isa ho nts'etsopele ea liphekolo tse ncha, electrophoresis e thehiloeng ho polyacrylamide e ntse e tsoela pele ho theha tsoelo-pele ea mahlale.Ha theknoloji e ntse e hatela pele, mohlolo ona oa maiketsetso o ka 'na oa fetoha, oa ntlafatsa kutloisiso ea rona ea liprotheine le mesebetsi ea tsona e mengata.